Molecular Biology Laboratory Skills Workshop: DNA Extraction & PCR

This workshop is part of the STEM Research Skills Workshop Series. 

Join Dr. Demetra Skaltsas for the two-day Molecular Biology Laboratory Skills Workshop, where we take you on a journey from mastering the basics of DNA extractions to becoming familiar with PCR techniques. This comprehensive workshop is designed to equip students with essential laboratory skills and techniques necessary for success in molecular biology research and related disciplines.

In the DNA Extractions: Basics module, students will learn fundamental skills such as pipetting techniques, proper handling methods, and safety protocols associated with heat blocks and centrifuges. Additionally, participants will gain insights into assessing DNA quality and quantity using nanodrop and Qubit technologies, as well as differentiating between quick extraction methods and others.

Moving on to the PCR module, participants will delve deeper into the intricacies of polymerase chain reaction (PCR). This series goes beyond simply performing PCR and running electrophoresis gels. By the end of the series, students will be proficient in various skills including record-keeping, aseptic techniques, and troubleshooting common issues encountered during PCR experiments.

There will be an opportunity for participants to take a proficiency test and gain a certificate stating they received training in micropipetting, DNA extraction, sterile technique, PCR, and gel electrophoresis. It will be offered on the following days/times: 

The workshop will be offered on the following days/times: 

  • Option 1: Saturday, October 19, 2024 - Sunday, October 20, 2024; 9am-5pm each day (breaks included & food provided)
  • Option 2: Saturday, October 26, 2024 - Sunday, October 27, 2024; 9am-5pm each day (breaks included & food provided)
  • Option 3: Saturday, November 16, 2024 - Sunday, November 17, 2024; 9am-5pm each day (breaks included & food provided)
  • Option 4: Saturday, November 23, 2024 - Sunday, November 24, 2024; 9am-5pm each day (breaks included & food provided)

Register HERE: https://uarizona.co1.qualtrics.com/jfe/form/SV_6yvgV9xaZLciFOm

Highlights of the workshop include: 

  • Pipetting mastery
  • Aseptic technique
  • DNA isolation, amplification, visualization, and interpretation
  • Primer elutions and dilutions
  • Gel electrophoresis
  • Polymerase Chain Reaction
  • Time management strategies
  • Troubleshooting in research

This series will be offered  multiple times, ensuring flexibility for both introductory sessions and practice sessions for those seeking to refresh their skills or enhance proficiency. Whether you're a beginner or looking to refine your molecular biology techniques, this workshop provides the foundation for success in the laboratory and beyond. Join us in mastering the art of molecular biology experimentation!

Questions? Email Courtney Leligdon at cleligdon@arizona.edu. 

Interested in learning about other Research Skills Workshops? Visit ur.arizona.edu/content/stem-skills-workshop!